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. 2023 May 29;13:8659. doi: 10.1038/s41598-023-35875-1

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Screening the siRNA knockdown library for regulators of NKX6.1 expression in pancreatic endoderm. (A) A schematic diagram of the procedures used to differentiate pancreatic endoderm cells from iPSCs. A activin A; C CHIR99021; Y Y-27632; K KGF; N Noggin; KC KAAD-cyclopamine; TT TTNPB; E EGF; Ni nicotinamide; TP TPB. (B) A schematic diagram showing NKX6.1⁺ relative scores for each gene in the screening. (C) A scatterplot showing NKX6.1⁺ relative scores of each siRNA from the 1st screen. (D) The list of 29 hit genes in the 1st screening. (E) A scatterplot showing NKX6.1⁺ relative scores of each siRNA from the 2nd screen. (F) Validation of 7 hit genes using siRNAs with different sequences. The data from four independent experiments are presented as the mean ± SD (n = 4). **p < 0.01 by one-way ANOVA with Dunnett’s test for comparison with no siRNA.