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. 2023 May 29;13:8659. doi: 10.1038/s41598-023-35875-1

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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HHEX contributes to the generation of pancreatic endoderm. (A) The expression of HHEX during differentiation from the undifferentiated state to pancreatic endoderm cells with (solid line) or without re-seeding on Stage 3 day 2 (dotted line). (B) A representative flow cytometer plot showing the transfection efficiency of pCXLE-eGFP into pancreatic endoderm cells by electroporation. (C) Expressions of HHEX, NKX6.1 and PTF1A in Stage 4 day 1 (day 11; left panels) and Stage 4 day 4 (day 14; right panels) cells overexpressing HHEX on Stage 4 day 0 (day 10). (D) Representative flow cytometer plots showing NKX6.1⁺ cell percentage upon the overexpression of HHEX. The data from three independent experiments are presented as the mean ± SD (n = 3). *p < 0.05 and **p < 0.01 by two-tailed Student's t-test.