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Syntaxin 7 is concentrated in the bilayered coat (A) WtGHR cells. Syntaxin 7 (10-nm gold) is largely restricted to the limiting membrane of EEs, where it accumulates in the bilayered coated areas (arrows). (B) In HeLa cells, syntaxin 7 (10-nm gold) shows a similar staining. Bars, 200 nm.