Table 2.

Overview of the sample size, medium, and tolerated delay (transport time) that need to be taken into account during surgery in order to apply various methods to human cortical access tissue

Method	Size [mm]	Transfer medium	Time [min]	Reference
Single sample
Electrophysiology electron microscopy	5–7	NMDG aCSF	15	[15, 38]
Stimulated Raman histology	1–4	Moist gauze	20	[8, 21, 35]
Large sample
Organotypic cell culture	7–20	Prep. medium	5	[7, 23, 30]
Spatial transcriptomics	6		5–10	[25, 33]
Single-cell transcriptomics	20–40		20	[24]
Bulk transcriptomics	1–2		5–20	[7]
Mass spectroscopy	1–2		5–10	[17]