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. 2023 Apr 3;75(3):570–584. doi: 10.1007/s43440-023-00480-6

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© The Author(s) 2023, corrected publication 2023

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Fig. 1 — MPM cell lines are resistant to ADI-PEG20 upon co-culture with macrophages. Three ASS1-negative mesothelioma cell lines A 2591, B MSTO, and C JU77 were cultured alone and with macrophages, either with or without direct cell contact, and in the presence and absence of ADI-PEG20 (750 ng/ml). Two independent experiments were performed for each cell line in triplicate (n = 3). Viability was assessed at 4 days by flow cytometry. Bars show mean and standard deviation with statistical significance shown for the DAPI(low)/annexin V(low) cells only, ****p < 0.0001; Two-way ANOVA with post hoc Tukey’s test. Compared with the tumor (control) group, co-culture significantly increased MPM cell viability under ADI-PEG20: 2591 (F_3,48 = 1481, df = 3); MSTO (F_3,48 = 1193, df = 3); and JU77 F_5,24 = 11,533, df = 3)