|
Gene
|
Sequence
|
Length
|
GC%
|
Tm
|
ESR1 F
|
5
CAAGAGAAGTATTCAAGGACATAACG 3
|
26 |
38.46 |
58.10 |
ESR1 R
|
5
GTATCCCACCTTTCATCATTCCC 3
|
23 |
47.83 |
58.85 |
PGR F
|
5
GTGGTCTAAATCATTGCCAGG 3
|
21 |
47.62 |
56.94 |
PGR R
|
5
CTTTCATCCGCTGTTCATTTAG 3
|
22 |
40.91 |
55.80 |
PGRB F
|
5
CCTTGTTGTATTTGTGCGTGTGG3
|
23 |
47.83 |
61.09 |
PGRB R
|
5
TCCACTGCCCCCTCACTAAAAC 3
|
22 |
54.55 |
62.21 |
GAPDH F
|
5
CAACTACATGGTCTACATG 3
|
19 |
42.11 |
50.23 |
GAPDH R
|
5
CTCGCTCCTGGAAGATG 3
|
17 |
58.82 |
53.83 |
qRT-PCR: Quantitative real-time polymerase chain reaction, GC: Guanine cytosine, Tm: Temperature, ESR1: Estrogen receptor 1, PGR: Progesterone receptor, PGRB: Progesterone receptor B, GAPDH: Glyceraldehyde-3-phosphate dehydrogenase |