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. 2023 May 8;21(4):343–354. doi: 10.18502/ijrm.v21i4.13273

Table 1.

Primer sequences used for qRT-PCR


Gene Sequence Length GC% Tm
ESR1 F 5 ' CAAGAGAAGTATTCAAGGACATAACG 3 ' 26 38.46 58.10
ESR1 R 5 ' GTATCCCACCTTTCATCATTCCC 3 ' 23 47.83 58.85
PGR F 5 ' GTGGTCTAAATCATTGCCAGG 3 ' 21 47.62 56.94
PGR R 5 ' CTTTCATCCGCTGTTCATTTAG 3 ' 22 40.91 55.80
PGRB F 5 ' CCTTGTTGTATTTGTGCGTGTGG3 ' 23 47.83 61.09
PGRB R 5 ' TCCACTGCCCCCTCACTAAAAC 3 ' 22 54.55 62.21
GAPDH F 5 ' CAACTACATGGTCTACATG 3 ' 19 42.11 50.23
GAPDH R 5 ' CTCGCTCCTGGAAGATG 3 ' 17 58.82 53.83
qRT-PCR: Quantitative real-time polymerase chain reaction, GC: Guanine cytosine, Tm: Temperature, ESR1: Estrogen receptor 1, PGR: Progesterone receptor, PGRB: Progesterone receptor B, GAPDH: Glyceraldehyde-3-phosphate dehydrogenase