Figure 2.
LFY protein forms biomolecular condensates in planta
(A) Diffusion coefficients of condensates in root cells from 5-day-old 35S::LFY-YFP seedlings. n = 13.
(B) Time course analysis of condensate fusion in root cells from 5-day-old 35S::LFY-YFP seedlings.
(C) Analysis of the shape (top), size (center), and intensity enrichment relative to the nuclear intensity (bottom) of LFY-YFP condensates in root cells from 5-day-old 35S::LFY-YFP seedlings. n = 13.
(D) FRAP recovery curves after photobleaching of whole condensates in roots from 5-day-old 35S::LFY-YFP seedlings. Top: representative LFY condensate during an FRAP experiment. Scale bar = 1 μm. Bottom: FRAP recovery curve. n = 9. Error bars represent standard deviations.
(E) Half-FRAP recovery curves after photobleaching condensates in N. benthamiana leaf cells transiently expressing 35S::LFY-mCit. Top: representative LFY condensate during a half-FRAP experiment. Scale bar = 1 μm. Bottom: Half-FRAP curves corresponding to the normalized recovery of the fluorescence in the bleached half (green) and the normalized loss of fluorescence in the non-bleached half (purple). The normalized intensity decrease (dip) in the non-bleached half amounted to 27% and was significantly larger than the dip for a freely diffusing protein (n = 9; p-value = 5·10−9, one-sided t-test).37 The same curves without the correction of immobile fractions are shown in the inset. Error bars represent standard errors of the mean.