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. 2023 May 15;26(6):106880. doi: 10.1016/j.isci.2023.106880

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© 2023 The Author(s)

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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UFO interacts with LFY in the nucleus and in cytoplasmic foci

(A) Transient expression of 35S::UFO-mCh in N. benthamiana leaves. Scale bar = 10 μm. Arrows indicate examples of cytoplasmic foci. Insets with higher magnification are shown.

(B) Transient coexpression of 35S::LFY-mCit and 35S::UFO-mCh in N. benthamiana leaves. mCit and mCh are false colored in green and magenta for better visualization of colocalization. Scale bar = 10 μm. Arrows indicate examples of cytoplasmic foci. Insets with higher magnification are shown.

(C) Bimolecular functional complementation of LFY and UFO in N. benthamiana leaves. LFY oligomerization was used as positive control, and the absence of LFY-URI interactions as negative control. Scale bar = 10 μm. In all panels, the overlay between mCitrine/mCherry fluorescence channels and the transmission view are shown. Arrows indicate examples of cytoplasmic foci. Insets with higher magnification are shown.