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. 2023 May 14;26(6):106882. doi: 10.1016/j.isci.2023.106882

Figure 1.

Figure 1

HDAC8 expression is upregulated in the intestine of humans and mice with NEC

(A) Heatmaps of HDAC expression in control and NEC samples (human: control = 8, NEC = 8; mice: control = 3, NEC = 8).

(B) HDAC8 protein levels were measured by Western blotting in healthy control and NEC samples.

(C) HDAC8 expression was assessed using immunostaining performed on the sections of the terminal ileum in healthy control and NEC samples. Scale bar: 50 μm.

(D) qRT‒PCR was performed to assess the relative expression levels of HDAC8 in IECs isolated from humans and mice. ∗p < 0.05, ∗∗p < 0.01 (Mann–Whitney test).

(E) qRT‒PCR was performed to assess the relative expression levels of HDAC8 in FHs74Int and IEC6 cells. ∗∗p < 0.01, and ∗∗∗p < 0.001 (two-tailed Student’s t test).

(F) qRT‒PCR was performed to assess the relative expression levels of HDAC8 in LPLs isolated from humans and mice. ns means no significance (Mann–Whitney test).