Figure 4.

Analysis of pathways regulated by HDAC8 in NEC

(A) Volcano plots were generated to infer the overall distribution of differentially expressed genes between NEC and control groups (upper panel); volcano plot shows the differentially expressed genes after PCI34051 treatment in the NEC group (lower panel). Genes were analyzed by DEseq2 and identified based on the cut off criterion: p values < 0.05, and |log2fc| > 1.

(B) Venn diagrams showing the overlapping genes with genes downregulated in NEC and genes upregulated after PCI34051 treatment (upper panel); Venn diagrams showing the overlapping genes with genes upregulated in NEC and genes downregulated after PCI34051 treatment (lower panel).

(C) KEGG pathway enrichment results for the genes regulated by HDAC8 in NEC.

(D) RRHO maps comparing NEC vs. control group and PCI34051+ NEC vs. NEC group. Degree of significance is depicted in the color bar below the RRHO maps.

(E) Venn diagram displaying the overlap between genes downregulated in NEC and upregulated after PCI34051 treatment (upper panel); Venn diagram displaying the overlap between genes upregulated in NEC and downregulated after PCI34051 treatment (lower panel). The overlapping genes were obtained from RRHO analysis.

(F) Pathway enrichment analysis for the Figure 4E overlapping genes, which are similar with the results analyzed by DEseq2.