Synthetic melanin serial dilution

Standard	Final concentration/ μg/mL	Volume of melanin/ μL	Volume of 1 M NaOH /μL
A	100	100 from stock∗	900
B	75	600 from A	200
C	50	400 from B	200
D	25	250 from C	250
E	5	60 from D	240
F	0	0	130

∗When not in use, the synthetic melanin stock (100 μg/mL) should be stored at 4°C. Dispose of reagents after one month.