| Step | Reagent | Duration |
|---|---|---|
| 1 | Hematoxylin | 2 min |
| 2 | Running water | 2 min |
| 3 | Scotts’ bluing water | 2 min |
| 4 | Running water | 2 min |
| 5 | Eosin Y Aqueuos | 20 s |
| 6 | 70% Ethanol | 1 dip |
| 7 | 80% Ethanol | 5 s |
| 8 | 90% Ethanol | 5 s |
| 9 | 100% Ethanol | 3 min |
| 10 | 100% Ethanol | 3 min |
| 11 | Xylene | 3 min |
| 12 | Xylene | 3 min |
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