REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Recombinant Anti-Cytokeratin 14 antibody [EPR17350] - Cytoskeleton Marker (ab181595) Dilution: 1:700	Abcam, UK	ab181595
Cytokeratin 10 Monoclonal Antibody (DE-K10) Dilution: 1:200	Thermo Fisher Scientific, USA	# MA5-13705
Anti-Melanoma Associated Antigen 100+ / 7 kDa antibody [NKI/beteb] (ab34165) Dilution: 1:100	Abcam, UK	ab34165
Anti-TRP1 Antibody (G-9): sc-166857 Dilution: 1:100	Santa Cruz Biotechnology, USA	sc-166857
Human CD117/c-kit Antibody Dilution: 1:100	R&D Systems, USA	AF332
Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150073) Dilution: 1:1000	Abcam, UK	ab150073
Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 594) (ab150076) Dilution: 1:1000	Abcam, UK	ab150076
Donkey Anti-Mouse IgG H&L (Alexa Fluor® 647) (ab150107) Dilution: 1:1000	Abcam, UK	ab150107
Donkey Anti-Goat IgG H&L (Alexa Fluor® 647) (ab150131) Dilution: 1:1000	Abcam, UK	ab150131
Recombinant Anti-Melanoma gp100 antibody [EP4863(2)] Dilution: 1:100	Abcam, UK	ab137079
Chemicals, peptides, and recombinant proteins
Sodium hydroxide	Cica-Web, Kanto Chemical Co., Inc, Japan	37184–00
Paraformaldehyde, powder, 95%	Sigma-Aldrich, USA	158127-500G
Hematoxylin solution, Harris	Sigma-Aldrich, USA	HHS16-500ML
Eosin Y solution, aqueous	Sigma-Aldrich, USA	HT110232-1L
Gelatin solution bioreagent	Sigma-Aldrich, USA	G1393-100ML
10× Phosphate buffered saline (PBS), biotechnology grade, 1L	1ST BASE/ Axil Scientific, Singapore	BUF-2041-10×1L
Dulbecco’s Phosphate-Buffered Saline (DPBS), 1×, w/o Ca & Mg, 500mL	Thermo Fisher Scientific, USA	SH30028.02
Kertinocyte SFM, Combo	Thermo Fisher Scientific, USA	17005042
Medium 254 500ML	Thermo Fisher Scientific, USA	M254500
HMGS−2 PMA−FREE 5ML	Thermo Fisher Scientific, USA	S0165
Trypsin-EDTA (0.25%), phenol red, 100 mL ∗VS.∗	Thermo Fisher Scientific, USA	25200056
Trypsin-EDTA (0.05%), phenol red, 100 mL ∗VS.∗	Thermo Fisher Scientific, USA	25300054
Triton™ X-100	Merck, Germany	T8787-100ML
SDS solution, molecular biology grade (10% w/v)	Promega, USA	V6551
CnT-PRIME Epithelial Culture Medium	CELLnTEC, Switzerland	CnT-PR
DMEM, high glucose	Thermo Fisher Scientific, USA	11965092
cOmplete™ Lysis-M	Merck, Germany	04719956001
cOmplete™, Mini, EDTA-free Protease Inhibitor Cocktail	Merck, Germany	04693159001
Corning Collagen I, Rat Tail, 100 mg	Corning Inc, USA	354236
Target retrieval solution, pH 9 (10×)	Agilent Technologies, USA	S236784-2
Prolong Diamond Antifade Mount	Thermo Fisher Scientific, USA	P36970
Experimental models: Cell lines
Human Epidermal Melanocytes, neonatal, moderately pigmented donor, (HEMn-MP)	Thermo Fisher Scientific, USA	C1025C
Human Keratinocytes N/TERT -1	Brigham and Women’s Hospital, Department of Dermatology James G. Rheinwald lab.	CVCL_CW92
hTERT-immortalized human normal dermal fibroblast	Gift from Dr Oliver Dreesen’s lab	Gift from Dr Oliver Dreesen’s lab
Software and algorithms
ImageJ	Schneider et al.	https://imagej.nih.gov/ij/
Other
Microplate, 96 well, PS, F-Bottom, Clear	Greiner Bio-One, Austria	655101
ThinCert Cell Culture Insert for 12-well plates, TC, sterile, translucent membrane (PET), pore diameter: 0,4 μm, 4 multiwell plates/box, 48 ThinCert inserts/box	Greiner Bio-One, Austria	665640
ThinCert plate, 12 well, PS, lid, sterile, single packed	Greiner Bio-One, Austria	665110
SEFAR Lab Pak (NITEX)	Sefar AG, Switzerland	03-70/33