Table 1.
Size selection during the early phase of fluid phase endocytosis
| Dex-TRITC (Fluorescence intensity) | LY (Fluorescence intensity) | Ratio (A. U.) | 70 kDa-Dex-TRITC alone (A. U.) | |
|---|---|---|---|---|
| AX2 | 1.67 ± 0.34 (100%) | 3.79 ± 0.66 (100%) | 0.44 ± 0.02 | 100% |
| AX2 + CytA | 0.93 ± 0.14 (56%) | 2.74 ± 0.45 (72%) | 0.34 ± 0.02 | 90% |
| chc− | 0.19 ± 0.03 (11%) | 3.04 ± 0.67 (80%) | 0.06 ± 0.01 | 37% |
| chc−+ CytA | 0.17 ± 0.02 (10%) | 1.62 ± 0.23 (43%) | 0.11 ± 0.01 | 50% |
| Sizes | >9 nm ⊘ | ∼1 nm ⊘ | >3 nm ⊘ |
Effect of absence of clathrin heavy chain and disruption of F-actin. The values shown were taken after 15 min of co-endocytosis of 2,000,000-kDa dextran-TRITC and LY (the fluorescence intensity ratio of TRITC to LY in medium was ∼0.21). The values for the uptake of 70-kDa dextran are from the 15-min time point of the curves presented in Figure 5A. As a reference, the fluid phase markers used for EM, HRP, and BSA-Au were ∼2.4 nm and ∼14 nm in diameter, respectively. A.U., arbitrary units.