Figure 2.

Adhesion of wild-type (wt) and fla10 gametes at the permissive and restrictive temperatures. (A) Wild-type (left) and fla10 mt− gametes (right) maintained at 21°C (▵) or preincubated at 32°C for 40 min (▴) were mixed with imp1 mt+ gametes at 21 or 32°C, respectively, and cell adhesion was determined by use of an electronic particle counter. (B) fla10 gametes maintained at 21°C (left) or preincubated at 32°C (right) were mixed with wild-type mt+ gametes at 21 or 32°C, respectively, fixed with osmium fumes (Hunnicutt and Snell, 1991), and examined by phase contrast microscopy.