Figure 3.

Loss and restoration of flagellar agglutinins in fla10 gametes at the restrictive temperature. (A) Cell adhesion from the samples in Figure 2A was followed for an additional 30 min. Only the fla10 samples at 32°C lost their adhesiveness. wt, wild type. (B) fla10 gametes preincubated at 32°C for 40 min were mixed with imp1 mt+ gametes at 32°C at T₀, and cell adhesion was determined using an electronic particle counter (▵). After 30 min, when the majority of the cells had deadhered, a portion of the sample was incubated with dibutyryl cAMP (▴). ●, cell adhesion results from a control sample in which fla10 gametes that had been preincubated at 32°C for a total of 70 min were mixed with imp1 gametes.