Figure 6.

Changes in cAMP levels during adhesion of wild-type (wt) and fla10 gametes at the permissive and restrictive temperatures. Wild-type (A) and fla10 mt− (B) gametes maintained at 21°C or preincubated at 32°C for 40 min were mixed with imp1 gametes at the indicated temperatures, and at the indicated times after mixing, samples were analyzed for cAMP. C, ratios of cAMP present at 32/21°C, 1 min after mixing wild-type or fla10 gametes from the panel A/B experiment and from two separate experiments, in one of which the mixing was carried out in the presence of the phosphodiesterase inhibitor, papaverine (Pasquale and Goodenough, 1987; Hunnicutt et al., 1990).