Table 1.
Cryo-EM data statistics
| Human PRPS2-short filament | |
| EM equipment | Titan Krios |
| Detector | K3 camera |
| Magnification | 22,500x |
| Voltage (kV) | 300 |
| Electron exposure ((e–/Å2)) | 60 |
| Defocus range(μm) | − 1.0 to − 2.5 |
| Pixel size(Å) | 1.06 |
| Symmetry imposed | D3 |
| Number of collected movies | 2403 |
| Initial particle images (no.) | 681,672 |
| Final particle images (no.) | 140,303 |
| Refinement | |
| EMDB ID | EMD-33883 |
| PDB code | 7YK1 |
| Initial model used (PDB code) | - |
| Map resolution (Å) | 3.1 |
| FSC threshold | 0.143 |
| Map resolution range (Å) | 2.9–3.9 |
| Map sharpening B-factor(Å2) | -74 |
| Model composition | |
| Non-hydrogen atoms | 14,364 |
| Protein residues | 1836 |
| Ligands | ADP,Pi,Mg |
| Ions | 12 |
| B factors(Å2) | |
| Protein | 58 |
| Ligand | 67 |
| R.m.s. deviations | |
| Bond lengths (Å) | 0.005 |
| Bond angles (°) | 0.690 |
| Validation | |
| MolProbity score | 1.70 |
| Clashscore | 5.53 |
| Poor rotamers (%) | 0.13 |
| Ramachandran plot | |
| Favored (%) | 93.98 |
| Allowed (%) | 5.24 |
| Disallowed (%) | 0.77 |