Table 2.
Summary of mutant phenotypes
| Mutant | ADP-Ribose activity (% of wt) | Deacetylase activitya | HM silencing | Telomere silencing | rDNA recombinations rate (%) | ChIP at telomeres |
|---|---|---|---|---|---|---|
| wt | 100 | 100 | + | + | 0.1 | + |
| Δsir2 | N/A | N/A | − | − | 1.2 | − |
| Thr-261 | 4 | 5 | + | + | 0.1 | + |
| Gly-270 | 7 | 21 | + | ± | 0.1 | ± |
| Ile-271 | 8 | 9 | + | − | 0.2 | − |
| Phe-274 | ND | 9 | − | − | 0.9 | − |
| Arg-275 | 100 | 78 | − | − | 1.0 | − |
| Asn-345 | 0 | 0.3 | − | − | 1.2 | − |
| Asp-347 | 0 | 0.4 | − | − | ND | − |
| His-364 | ND | 0.4 | − | − | ND | − |
N/A, not applicable; ND, not determined; wt, wild type.
a
As a percentage of wild-type minus the pET negative control.