| Antibodies |
| Latent Associated Protein (LAP) antibody |
Novus |
Cat #NBP2-22114SS; RRID:AB_2909813 |
| TGFβ |
Cell Signaling Technology |
Cat #3711; RRID:AB_2063354 |
| Latent Binding TGFβ Protein (LTBP) |
Abcam |
Cat #ab78294; RRID:AB_1952060 |
| pIκB |
Cell Signaling Technology |
Cat #2859T; RRID:AB_561111 |
| IκB |
Cell Signaling Technology |
Cat #4814T; RRID:AB_390781 |
| tubulin |
Sigma |
Cat #T4026; RRID:AB_477577 |
| Twist1 |
Thermo Fisher Scientific |
Cat #PA5116093; RRID:AB_2900727 |
| Zeb1 |
Thermo Fisher Scientific |
Cat #PA595862; RRID:AB_2807664 |
| Snail |
Thermo Fisher Scientific |
Cat #PA5115940; RRID:AB_2900574 |
| E-cadherin |
Cell Signaling Technology |
Cat #3195S; ID UniProt : P12830
|
| Phospho Smad2 |
Cell Signaling Technology |
Cat #3108; ID UniProt : Q15796
|
| Smad2 |
Cell Signaling Technology |
Cat #3103; ID Uniprot : Q15796
|
| myeloperoxidase |
Dako |
Cat #A0398; RRID:AB_2335676 |
| Histone H2B |
Abcam |
Cat #ab52484; RRID:AB_1139809 |
| RFP (mcherry) |
Rockland |
Cat #600-401-379; RRID:AB_2209751 |
| ASC |
EMD Millipore |
Cat #04-147; RRID:AB_1977033 |
| ITGβ1 |
BD Biosciences |
Cat #555003; RRID:AB_395637 |
| ITGαv (clone RMV-7) |
Invitrogen |
Cat #14-0512-82; RRID:AB_467296 |
| MMP9 |
Abcam |
Cat #ab38898; RRID:AB_776512 |
| TGFβ |
Cell Signaling Technology |
Cat #3711; RRID:AB_2063354 |
| anti-myeloperoxidase antibody (used for mouse tissue) |
R & D Systems |
Cat #AF3667; RRID:AB_2250866 |
| anti-myeloperoxidase antibody (used for human tissue) |
R & D Systems |
Cat #MAB3174; RRID:AB_2250873 |
| anti-citrullinated histone H3 antibody (used for mouse and human tissue) |
Abcam |
Cat #ab5103; RRID:AB_304752 |
| anti-N-cadherin |
Abcam |
Cat #ab18203; ID Uniprot:P19022
|
| anti-Claudin1 |
Thermo Fisher Scientific |
Cat #71-7800; RRID:AB_2533997 |
| Donkey anti-Goat IgG secondary antibody, Alexa Fluor 647 |
Invitrogen |
Cat #A21445; RRID:AB_2535862 |
| Donkey anti-Rabit IgG secondary antibody, Alexa Fluor 568 |
Invitrogen |
Cat #A10042; RRID:AB_2534017 |
| ITGβ1 (clone Ha2/5) |
BD Biosciences |
Cat #555003; RRID:AB_395637 |
| ITGαv (clone RMV-7) |
Invitrogen |
Cat #14-0512-82; RRID:AB_467296 |
| CXCL5 |
R & D Systems |
Cat #Mab433; RRID:AB_2086587 |
| CXCL1 |
R & D Systems |
Cat #Mab453; RRID:AB_2087696 |
| IL-1β |
R & D Systems |
Cat #AF-401-NA; RRID:AB_416684 |
| anti-mouse Neutrophil Elastase |
Santa Cruz Biotechnology |
Cat #sc-9521; RRID:AB_2096537 |
| anti-human Neutrophil Elastase |
Santa Cruz Biotechnology |
Cat #sc-9520; RRID:AB_650173 |
| anti-DNA peroxidase conjugated |
Roche |
Cat#11774425001; RRID:AB_2909412 |
| CD4 (clone GK1.5) |
BD Biosciences |
Cat #612974; RRID:AB_2870246 |
| CD8a (clone REA601) |
Miltenyi Biotec |
Cat #130-120-806; RRID:AB_2752203 |
| TCR β (clone REA318) |
Miltenyi Biotec |
Cat #130-104-856; RRID:AB_2654019 |
| CD19 (clone T45-2342) |
Miltenyi Biotec |
Cat #130-112-042; RRID:AB_2655828 |
| CD45 (clone REA737) |
Miltenyi Biotec |
Cat #130-110-803; RRID:AB_2658224 |
| NKp46 (clone 29A1.4) |
BD Biosciences |
Cat #741029; RRID:AB_2740647 |
| F4/80 (clone T45-2342) |
BD Biosciences |
Cat #743281; RRID:AB_2741399 |
| CD11b (clone REA754) |
Miltenyi Biotec |
Cat #130-113-808; RRID:AB_2751173 |
| CD11c (clone REA754) |
Miltenyi Biotec |
Cat #130-110-837; RRID:AB_2654705 |
| Ly6C (clone REA796) |
Miltenyi Biotec |
Cat #130-111-920; RRID:AB_2652814 |
| Ly6G (clone 1A8) |
BD Biosciences |
Cat #561236; RRID:AB_10611860 |
| CD107a (clone 1D4B) |
BD Biosciences |
Cat #565533; RRID:AB_2739285 |
| CD326 |
Biolegend |
Cat #118214; RRID:AB_1134102 |
| anti-Ly6G antibody (clone 1A8) |
BioXCell |
Cat #BP0075-1; RRID:AB_1107721 |
| IgG for antiLy6G antibody (clone 2A3) |
BioXCell |
Cat #BP0089; RRID:AB_1107769 |
| anti-IL-1β antibody (clone B122) |
BioXCell |
Cat #BE0246; RRID:AB_2687727 |
| IgG for anti-IL-1β antibody |
BioXCell |
Cat #BE0091; RRID:AB_1107773 |
| anti-TGFβ antibody (clone 1D11.16.8) |
BioXCell |
Cat #BE0057; RRID:AB_1107757 |
| IgG for anti-TGFβ antibody (clone MOPC-21) |
BioXCell |
Cat #BE0083; RRID:AB_1107784 |
| Biological samples |
|
|
| Human plasma samples from metastatic breast cancer patients |
Institut Curie, Paris, France |
Table S5
|
| Paraffin-embeded breast cancer tissue |
University of Southern Denmark |
Table S6
|
| Chemicals, peptides, and recombinant proteins |
| Cisplatin |
Sigma |
Cat #C2210000 |
| Doxorubicin |
Sigma |
Cat #D1515 |
| Cyclophosphamide |
Sigma |
Cat #C0768 |
| PAD4 inh. |
Cayman Chemical |
Cat #17488 |
| CXCR2 inh. |
Selleckchem |
Cat #SB225002 |
| NLRP3 inh. (MCC950) |
EMD Millipore |
Cat #5.38120.0001 |
| MMP9 inh. (in vivo) |
Medchem Express |
Cat #HY-12354 |
| TGFβR1 inh. |
Selleckchem |
Cat #SB431542 |
| ITG αVβ1 inh. |
Medchem Express |
Cat #HY-100445A |
| DNAse I |
Sigma |
Cat #04536282001 |
| HBSS 1X without Ca2+/Mg2+ |
Gibco |
Cat #14175-053 |
| ACK |
Gibco |
Cat #A10492-01 |
| Percoll |
GE Healthcare |
Cat #17-0891-02 |
| serum-free DMEM (Dulbecco’s Modified Eagle Medium) |
Gibco |
Cat #21969-035 |
| Dharmafect 3 |
Dharmacon |
Cat #T-2003-02 |
| Opti-MEM |
Gibco |
Cat #31985-047 |
| Cytiva HyClone™ Sérum FetalClone™ II (FCII) |
Cytiva |
Cat #SH3006603 |
| Matrigel |
Corning |
Cat #356234 |
| Luciferin |
Goldbio |
Cat #Luck-1G |
| Phorbol 12-myristate 13-acetate (PMA) |
Sigma |
Cat #P1585 |
| Recombinant mouse complement 5a (C5a) |
R & D Systems |
Cat #2150-C5/CF |
| Recombinant IL-1β |
Peprotech |
Cat #211-11B |
| Recombiant TGFβ-1 |
Biolegend |
Cat #763102 |
| Caspase I inh. AC-YVAD-CMK |
abcam |
Cat #SML0429 |
| NF-kB inh. Bay 11-7082 |
Cayman Chemical |
Cat#10010266 |
| NE inh. Sivelestat |
Tocris |
Cat #3535 |
| MMP9 inh. 1 (in vitro) |
Calbiochem |
Cat #44278 |
| Cathepsin G inhibitor 1 |
EMD Millipore |
Cat #219372 |
| Myeloperoxidase inh. (4-Aminoebnzoic Acid hydrazide) |
Cayman Chemical |
Cat #14845 |
| RGD peptide |
abcam |
Cat #A8052 |
| Sytox green |
Thermo Fisher Scientific |
Cat #S7020 |
| Chemiluminescent horseradish peroxidase (HRP) substrate |
GE Healthcare |
Cat #RPN 2109 |
| RPMI |
Gibco |
Cat #31870-025 |
| Dispase |
Stem Cell |
Cat #07913 |
| Collagenase D |
Sigma |
Cat#11088866001 |
| Liberase DL |
Sigma |
Cat #05466202001 |
| Fc block |
BD Biosciences |
Cat#553142 |
| PFA |
Electron Microscopy Science |
Cat #15714 |
| Triton X-100 |
Thermo Fisher Scientific |
Cat #BB151-500 |
| Fc receptor blocker |
Innovex |
Cat #NB309 |
| Donkey serum |
Sigma |
Cat #D9663 |
| BSA |
Sigma |
Cat #A3294 |
| DAPI |
Thermo Fisher Scientific |
Cat #D1306 |
| Hematoxylin |
Thermo Fisher Scientific |
Cat #6765008 |
| Eosin |
Sigma |
Cat #HT110180 |
| Polybrene |
Sigma |
Cat #TR-1003-G |
| Puromycine |
Sigma |
Cat #P8833 |
| L-Glutamine |
Gibco |
Cat #25030 |
| Penicillin-streptomycin |
Gibco |
Cat #15140-122 |
| Critical commercial assays |
| 96-well Enzyme ImmunoAssay/Radio ImmunoAssay (EIA/RIA) |
Costar |
Cat #3590 |
| ELISA kit for TGF-β1 |
R & D Systems |
Cat #DY1679 |
| ELISA kit for Urea |
MyBiosource |
Cat #MBS2611085 |
| ELISA kit for creatinine |
MyBiosource |
Cat #MBS763433 |
| ELISA kit for IL-1β (for tissue analysis) |
R & D Systems |
Cat #MLB00C |
| ELISA kit for IL-1β (for CM analysis) |
Thermo Fisher Scientific |
Cat #88-7013-22 |
| ELISA kit for CXCL1/KC |
R & D Systems |
Cat #DY453 |
| ELISA kit for CXCL5 (LIX) |
R & D Systems |
Cat #P313546 |
| ATP determination kit |
Thermo Fisher Scientific |
Cat #A22066 |
| RNeasy Mini kit |
Qiagen, Turnberry Ln Valencia, CA |
Cat #217004 |
| Proteome Profiler Mouse XL Cytokine Array test |
R & D Systems |
Cat #ARY028 |
| PierceTM BCA Protein Assay Kit |
Thermofisher |
Cat #23225 |
| Illumina Stranded mRNA Prep |
Illumina |
Cat #20040532 |
| Deposited data |
| RNA-seq data |
This paper |
GEO: GSE218119
|
| Experimental models: Cell lines |
| 410.4 murine cancer cells |
Blazquez et al. 68
|
Obtained from Dr. Tobias Pukrop (University Hospital Regensburg, Regensburg, Germany) |
| PyMT murine cancer cells |
This paper |
Obtained from Dr. Bodu Liu (Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA) |
| TS/A murine cancer cells |
Merckmillipore |
Cat. # SCC177 |
| D2.OR murine cancer cells |
Barkan et al. 69
|
Gift from Dr. Jeffrey Green (National Institutes of Health) |
| D2.A1 murine cancer cells |
Barkan et al. 69
|
Gift from Dr. Jeffrey Green (National Institutes of Health) |
| 4T07 murine cancer cells |
Avgustinova et al. 70
|
Gift from Dr. Erik Sahai (The Francis Crick Institute, London, UK). RRID:CVCL_B383 |
| 4T1 murine cancer cells |
ATCC |
Cat. #CRL-2539 |
| 293T cells |
ATCC |
Cat. #CRL-3216 |
| Experimental models: Organisms/strains |
| Mouse Balb/c female |
Charles River |
Cat #028 |
| Mouse C57BL/6 female |
Charles River |
Cat #027 |
| Oligonucleotides |
| siRNA ON TARGET Plus Non-targeting pool target sequences: UGGUUUACAUGUCGACUAA; UGGUUUACAUGUUGUGUGA; UGGUUUACAUGUUUUCUGA; UGGUUUACAUGUUUUCCUA |
Dharmacon |
Cat #D-001810-10-05 |
| siRNA ON TARGET Plus mouse ccdc25 #1: -J-053396-09: GCAAGAUGAACAACGUUAA |
Dharmacon |
Cat #L-053396-01-0005 |
| siRNA ON TARGET Plus mouse ccdc25 #2: -J-053396-10: CCAUGUGUACCUACGAUUA |
Dharmacon |
Cat #L-053396-01-0005 |
| Additional primer sequences |
This paper |
Table S8
|
| Software and algorithms |
| ImageJ software |
ImageJ |
N/A |
| GraphPad Prism software version 7 |
GraphPad |
N/A |
| Cytexpert |
Beckman Coulter |
N/A |
| STAR |
Dobin et al. 71
|
N/A |
| FeatureCounts |
Liao et al. 72
|
N/A |
| DEseq2 |
Love et al. 73
|
N/A |
| ReactomePA R package |
Yu et al. 74
|
N/A |
| Other |
| 70μm nylon cell strainer |
BD Falcon |
Cat #352350 |
| 96-well plate |
Falcon |
Cat #353072 |
| Plate reader FLUOstar Omega |
BMG Labtech |
N/A |
| CytoFLEX |
Beckman Coulter |
N/A |
| Mounting media immunostaining |
Electron Microscopy Sciences |
Cat #17985-16 |
| Cytoseal 60 mounting media for histological staining |
Thermo Fisher Scientific |
Cat #8310-4 |
| Syringe 25 G |
Terumo |
Cat #AN*2516R1 |
| 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (antibodyTS) |
Thermo Fisher Scientific |
Cat #37615 |
| Superscript II reverse transcriptase |
Invitrogen, Carlsbad, CA |
Cat #18064-014 |
| Fast SYBR Green Master Mix |
Applied Biosystems, Foster City, CA |
Cat #18064-014 |
| Boyden chamber |
Corning |
Cat #351151 |
| 96-well Enzyme ImmunoAssay/Radio ImmunoAssay (EIA/RIA) plates |
Costar |
Cat #3590 |
| Cell-Free DNA BCT tubes |
Streck |
N/A |
