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Fig. 3. Demonstration of the shift of cof between three distinct DEP platforms. (A) Plot of DEP spectra predicted from cell properties by MyDEP. These results were used to find a static cof for melanoma cells (green area), which is plotted in (B) versus the CM factor corresponding to that frequency in the spectra shown in (A). Dynamic cofs are the observed transition of each cell line to a “pDEP response” in the 3DEP and CF-DEP platforms and are shown in the yellow and blue areas, respectively.