Fig. 6. Brightfield micrographs (A, B, G, H and I) showing cells captured in the DEP-BPE platform during a spike-in experiment. SK-MEL-28 cells spiked into healthy PBMCs were introduced at 100 nL min⁻¹ under an applied voltage of 16 V_pp at 50 kHz. After 2 h, the cells were labeled on chip. Fluorescence micrographs show captured SK-MEL-28 cells in (C and J) yellow (MCAM), (E and K) red (MCSP), (F and L) blue (Hoechst nuclear stain), and (D) green (CD45) channels. (I) Is a higher magnification brightfield image of an individually captured SK-MEL-28 cell. The scale bars in (A, B, G and H) are 100 μm and in (C–F) and (I–L) are 20 μm.