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GFP accumulation in regenerated central vacuoles of miniprotoplasts
| Reporter Protein | Time after Evacuolation
|
|
|---|---|---|
| 36 | 48–52 | |
| h | ||
| GFP5-Chi | 0% (n = 144) | 36% (n = 245) |
| Aleu-GFP6 | 97% (n = 161) | 84% (n = 208) |
Large CV started to become visible after 24 to 36 h. The percentage of fluorescent CV was calculated for all cells showing any green fluorescence.
