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. 2023 May 27;15(1):2217964. doi: 10.1080/19420862.2023.2217964

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© 2023 Philochem AG. Published with license by Taylor & Francis Group, LLC.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The terms on which this article has been published allow the posting of the Accepted Manuscript in a repository by the author(s) or with their consent.

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Immunofluorescence staining of a colon tumor, healthy colon, lung tumor, healthy lung, pancreas tumor, and healthy pancreas with the antibodies F4, Sm3E, and KSF. Strong green staining is present in the colon tumor and lung tumor with antibodies F4 and Sm3E and in slightly lower intensity in the pancreas tumor. No green staining is present with KSF and in all healthy organs. — Immunofluorescence analysis of human cancer sections. Human tissue microarrays with cancer sections and corresponding healthy controls were analyzed by immunofluorescence analysis with F4, the positive control Sm3E, and an irrelevant isotype control (KSF). Antibody binding was detected in green. Blood vessels were detected with CD31 staining (red) and nuclei with DAPI (blue). 20× magnification, scale bars = 100 μm.