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. 2023 May 29;20(1):257–271. doi: 10.1080/15476286.2023.2217392

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© 2023 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The terms on which this article has been published allow the posting of the Accepted Manuscript in a repository by the author(s) or with their consent.

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Figure 5. — NOL7 is required to maintain mature 18S rRNA levels in human tissue culture cells.

A. NOL7 is required for production of the normal 28S/18S mature rRNA ratio in MCF10A cells. Agilent BioAnalyzer analysis of ratio of mature 28S to 18S rRNAs from MCF10A cells depleted of NOL7. 3 biological replicates, plotted mean ± SD. Data were analysed by Student’s t-test relative to siNT negative control, * p ≤ 0.05.

B-C. NOL7 is small subunit (18S) specific in its role in rRNA maturation in MCF10A cells.

B. NOL7 is required for the production of the 18S rRNA. Agilent BioAnalyzer analysis of percent of overall RNA levels for 18S rRNA in MCF10A cells depleted of NOL7. 3 biological replicates, plotted mean ± SD. Data were analysed by Student’s t-test relative to siNT negative control, * p ≤ 0.05.

C. NOL7 is not required for the production of the 28S rRNA. Panel as above (B) except that the large subunit (28S) rRNA was measured.

D. siNOL7 increase in 28S/18S mature rRNA ratio can be rescued by introduction of an si-resistant version of NOL7 in Hela cells. Agilent BioAnalyzer analysis of ratio of mature 28S to 18S rRNAs upon NOL7 depletion and si-resistant rescue with either empty vector (HA EV) or HA-tagged NOL7 (HA NOL7) in HeLa cells. 3 biological replicates, plotted mean ± SD. Data were analysed by Student’s t-test relative to siNT negative control, * p ≤ 0.05, ns = not significant.

E-F. siNOL7 small subunit (18S) specific role in rRNA maturation can be rescued by introduction of an si-resistant version of NOL7 in HeLa cells.

E. siNOL7 decreases in 18S rRNA levels can be rescued by introduction of an si-resistant version of NOL7. Agilent BioAnalyzer analysis of percent of overall RNA levels upon NOL7 depletion and si-resistant rescue with either empty vector (HA EV) or HA-tagged NOL7 (HA NOL7) in HeLa cells. 3 biological replicates, plotted mean ± SD. Data were analysed by Student’s t-test relative to siNT negative control, * p ≤ 0.05, ns = not significant.

F. siNOL7 and si-resistant version of NOL7 rescue do not change 28S levels. Panel as above in (E) except that large subunit (28S) rRNA was measured.