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. 2023 May 27;18(1):2217033. doi: 10.1080/15592294.2023.2217033

Figure 5.

Figure 5.

METTL3 modulated m6A modification of lnc KCNQ1OT1 to facilitate DOX resistance of DOX-resistant BC cells.Note: a, qRT-PCR was applied to examine METTL3 expression in MCF10A, MCF-7, MDA-MB-231, MCF‐7/DOX, and MDA-MB-231/DOX cells. si-METTL3 or oe-METTL3 was transfected into DOX-resistant BC cells. b, qRT-PCR was performed to measure METTL3 and lnc KCNQ1OT1 expression. c, qRT-PCR was applied to detect the stability of lnc KCNQ1OT1. d, MeRIP‐qPCR was applied to test lnc KCNQ1OT1 expression of m6A modification. e, RIP assay was adopted to evaluate the enrichment of lnc KCNQ1OT1 by anti-METTL3 in MCF‐7/DOX and MDA-MB-231/DOX cells. si-METTLE3 alone or along with oe-lnc KCNQ1OT1 were transfected into DOX-resistant BC cells. f, cell viability was determined by MTT. g, cell proliferation was detected using clone formation. h, the apoptosis rate and cell cycle were measured with flow cytometry. i, lnc KCNQ1OT1, miR-103a-3p, and MDR1 expressions were tested using qRT-PCR. j, western blot was performed to measure MDR1, cyclin D1, Bax, and Bcl-2 expressions.