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. 2023 Mar 20;136(9):1098–1110. doi: 10.1097/CM9.0000000000002201

Figure 7.

Figure 7

MiR-140-3p inhibits ovarian cancer progression via inhibiting SNAI2. SKOV3 and CAOV-3 cells were pre-transfected and allocated into four groups: mimics-NC + vector group (cells transfected with empty plasmid), miR-140-3p mimics group (cells transfected with miR-140-3p mimics), SNAI2 group (cells transfected with pcDNA3.1-SNAI2), and miR-140-3p mimics + SNAI2 group (cells transfected with miR-140-3p mimics + pcDNA3.1-SNAI2). (A) CCK-8 assay was used to determine the proliferation of SKOV3 and CAOV-3 cells in different transfection groups. (B) Transwell assay was performed to investigate the changes of SKOV3 and CAOV-3 cell migration using crystal violet staining; scale bar = 100 μm. (C) Transwell assay was performed to research the changes of SKOV3 and CAOV-3 cell invasion using crystal violet staining; scale bar = 100 μm. (D) Western blot assay was applied to detect EMT-related protein (E-cadherin, N-cadherin, Snail, and Vimentin) changes of SKOV3 and CAOV-3 cells. N = 3; ∗P < 0.05, †P < 0.01, compared with the mimics-NC + vector group. CCK-8: Cell Counting Kit-8; EMT: Epithelial-mesenchymal transition.