Fig. 5. Principal component analysis (PCA) of average Raman spectra for just the 4-hour time point showing the box plots and PC loadings for PC5 and PC1. Box plot compares control cells and 6 Gy irradiated cells for unsynchronised UVW cells (blue) and synchronised UVW cells (pink). Centre point of box represents median value, notches represent the 25th and 75th percentile, whiskers represent the 5th and 95th percentile and stars represent outliers. PC5 and PC1 loading from individual time point PCA comparing control and 6 Gy samples for the 24-hour time point following 6 Gy XBR exposure for unsynchronised and synchronised UVW cells. Statistical analysis was performed using a two-way ANOVA with Wilcoxon rank sum test at 99% confidence interval (p > 0.05 = ns (not significant), p < 0.01 = ** and p < 0.001 = ***).