Figure 1.

Elicitation of rapid intracellular ROS transients by purified TMV. A, Epidermal peels from N. tabacum cv Samsun NN were loaded with DCFH-DA and monitored using fluorometry. As a control for the ability of the tissue to mount a ROS transient, l-Arg was added. B, As in A, but epidermal peels were from N. tabacum cv Samsun nn. C, As in A, but the virus added was CMV. As a control for the ability of the tissue to mount an ROS transient, TMV virus was added. D, As in C, but virus added was CGMMV. E, Dose response curve of ROS elicitation versus virus concentration using TMV or CMV and peels from N. tabacum cv Samsun NN or N. tabacum cv Samsun nn. Maximal response was recorded by the addition of 5 mm H₂O₂. Error bars are the mean and se of five experiments.