Figure 3.

The sensitivity of TMV-elicited ROS to pharmacological agents and temperature. The increase in DCF fluorescence in tobacco epidermal cells for each treatment is shown as the percentage of maximal response after the addition of 5 mm H₂O₂. A, DCF increase after addition of TMV (100 ng) in the presence (+) of the indicated pharmacological agents; CAT, catalase (100 units mL⁻¹); K252a, (25 μm); O.A., okadaic acid (100 nm); DPI, diphenyleneiodonium (10 μm). B, ROS transients elicited by TMV (100 ng), cryptogein (25 ng mL⁻¹), and l-Arg (1 mm) were carried out at temperatures ranging from 15°C to 45°C. Results are the mean and se for three replicate experiments.