Figure 6.

Elicitation of ROS by pH-treated TMV CP. Fluorescence of epidermal tissue was monitored during a time course in which in-flight additions were made of pretreated TMV CP (100 ng). A, TMV CP were pretreated at pH 8.0 (3 h in 10 mm Tris buffer), followed by addition of l-Arg (1 mm). B, TMV CP were pretreated at pH 5.0 (3 h in 10 mm MES buffer). C, TMV CP was treated as in A, then neutralized to pH 6.5 (3 h) at either room temperature or 4°C. D, Elution pattern of CP fractions used in A and B. The TMV CP was pretreated at pH 5.0 or 8.0 for 4 h and then resolved on a FPLC column at the same pH as the pretreatment. Calculated molecular masses of the peaks are shown.