Figure 5.

Redistribution of Cx43 and Cx45 protein expression in HPV-infected cervical tissues (A). Representative images of normal cervical stratified epithelium stained with Cx43- and Cx45-specific antibodies (top left and right, respectively) and hematoxylin-eosin (HE) (bottom). Representative images of normal cervical stratified epithelium stained for Cx43 (top left) and Cx45 (top right). Insets show enlargement of Cx43 and Cx45 staining in the selected areas (white squares). Scale bars=100 µm and 20 µm for large tissue views and insets, respectively. Data are representative of 3-4 independent biopsies. (B) Representative images of HPV18-positive CIN1 biopsies stained with p16^INK4a (p16)-specific antibodies and with either Cx43- or Cx45-specific antibodies (top left and right, respectively). Hoechst was used for nuclei staining. HE staining is shown (bottom panels). Enlarged images from selected regions (white squares) are shown on the right. Scale bars=100 µm and 20 µm for large tissue views and insets. Data are representative of 2-3 independent biopsies.