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. Author manuscript; available in PMC: 2023 May 30.

Published in final edited form as: J Comp Neurol. 2022 Jun 10;530(16):2804–2819. doi: 10.1002/cne.25376

Double fluorescence of the GRP (green) and superficial layers markers (magenta) of Sp5C and cervical spinal dorsal horn in adult macaque monkeys. Many GRP-ir fibers were colocalized with the marker for peptidergic neurons CGRP (a–c) and substance P (d–f) in the Sp5C. GRP-ir fibers were not colocalized with the marker for nonpeptidergic neurons IB₄ in the Sp5C (g–i). Most GRP-ir fibers were colocalized with CGRP(j–l) and substance P (m–o) in the superficial layers of the cervical spinal cord. GRP-ir fibers were not colocalized with IB₄ (p–r) in the cervical spinal dorsal horn. Bars = 200 μm (i, r)

Double fluorescence of the GRP (green) and superficial layers markers (magenta) of Sp5C and cervical spinal dorsal horn in adult macaque monkeys. Many GRP-ir fibers were colocalized with the marker for peptidergic neurons CGRP (a–c) and substance P (d–f) in the Sp5C. GRP-ir fibers were not colocalized with the marker for nonpeptidergic neurons IB₄ in the Sp5C (g–i). Most GRP-ir fibers were colocalized with CGRP(j–l) and substance P (m–o) in the superficial layers of the cervical spinal cord. GRP-ir fibers were not colocalized with IB₄ (p–r) in the cervical spinal dorsal horn. Bars = 200 μm (i, r)