TABLE 1.
Primary antibodies used in this study
| Antigen | Description | Host species and type | Working dilution | Catalog information | RRID |
|---|---|---|---|---|---|
| GRP | Mouse neuromedin C (or GRP-10; GSHWAVGHLM) | Rabbit polyclonal IgG | 1:2,000 (IHC) 1:1,000 (IF) | AssayPro, 11081-05015 | AB_2571636 |
| CGRP | Synthetic rat α-CGRP, Clone 4901 | Mouse monoclonal IgG | 1:20,000 (IF) | Abcam, ab81887 | AB_1658411 |
| Substance P | Amino acid: 1–11 (RPKPQQFFGLM) of rat substance P | Guinea pig polyclonal IgG | 1:10,000 (IF) | Abcam, ab10353 | AB_297089 |
| GRPR | Amino acids: 200–294 of human GRPR | Rabbit polyclonal IgG | 1:1,000 (WB) | GeneTex, GTX100015 | AB_1240922 |
| FITC | FITC antibody conjugated to HRP | Sheep polyclonal IgG | 1:2,000 (WLB) | PerkinElmer, NEF710 | AB_2314403 |
Abbreviations. FITC, fluorescein isothiocyanate; HRP, horseradish peroxidase; IF, immunofluorescence; IHC, immunohistochemistry; WB, western blot; WLB, western ligand binding blot.