Overview of Epic Sciences’ single-CTC sWGS methodology in which nucleated cells are extracted from whole blood, plated onto slides, stained (CK, DAPI, and CD45), and scanned for CTCs using automated imaging software. Individual CTCs are isolated from the slides for DNA extraction and sWGS. CTC = circulating tumor cell; ctDNA = circulating tumor DNA; sWGS = shallow whole-genome sequencing.