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. Author manuscript; available in PMC: 2023 May 30.
Published in final edited form as: J Vis Exp. 2023 Jan 20;(191):10.3791/64001. doi: 10.3791/64001

Table 2:

Biotin Fill-In reagents

Reagent Amount
Restriction Buffer * (10×) 5.8 μL
10 mM dCTP 1.5 μL
10 mM dGTP 1.5 μL
10 mM dTTP 1.5 μL
0.4 mM biotin-14-dATP* 37.5 μL
Ultrapure water 0.2 μL
Klenow DNA polymerase (5 U/μL) 10 μL
TOTAL 58 μL
*

Note that changing enzymes may require different buffers and biotinylated dNTPs.