Table 3:
Ligation reagents
| Reagent | Amount |
|---|---|
| Ultrapure water | 243 μL |
| T4 ligation buffer (5×) | 240 μL |
| Triton X-100 (10%) | 120 μL |
| BSA (10 mg/ml) | 12 μL |
| T4 DNA ligase (1U/μL) | 50 μL |
| TOTAL | 665 μL |
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