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. Author manuscript; available in PMC: 2023 May 30.
Published in final edited form as: J Vis Exp. 2023 Jan 20;(191):10.3791/64001. doi: 10.3791/64001

Table 5:

Reagents and cycling parameters for removal of biotin from unligated ends

Reagent Amount
Hi-C DNA sample 5–15 μg
Pre-PCR Buffer (10×) 13 μL
1 mM dATP 3.25 μL
1 mM dGTP 3.25 μL
T4 DNA polymerase (3,000 U/mL) 13 μL
Ultrapure water to 130 μL
TOTAL 130 μL
Cycling parameters
Temperature Time
20 ºC 4 hours
75 ºC 20 minutes
4 ºC hold