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A-tailing reagents and temperatures
| Reagent | Amount |
| Hi-C library on beads (step 3.5.14) | 41 μL |
| Pre-PCR Buffer (10×) | 5 μL |
| dATP (10 mM) | 1 μL |
| Klenow Fragment (3→5’ exo-; 5 U/μL) | 3 μL |
| TOTAL | 50 μL |
| Temperature | Time |
| 37 ºC | 30 minutes |
| 65 ºC | 20 minutes |
| 4 ºC | hold |
