Table 9:

PCR Primers and Paired-End-adapter oligos with reagents annealing for annealing

Oligo	Sequence
PE1.0 primer	CAAGCAGAAGACGGCATACGAGA*T
PE2.0 primer	AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACG*A
Adapter-Index01	/5PHOS/GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGTCTTCTGCTTG
Adapter-Index02	/5PHOS/GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTG
Adapter-Index03	/5PHOS/GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCCGTCTTCTGCTTG
Adapter-Index04	/5PHOS/GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCTTG
Adapter-Index05	/5PHOS/GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTCTTCTGCTTG
Adapter-Index06	/5PHOS/GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTCTTCTGCTTG
Adapter-Index07	/5PHOS/GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGTCTTCTGCTTG
Adapter-Index08	/5PHOS/GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCCGTCTTCTGCTTG
Adapter-Index09	/5PHOS/GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTTCTGCTTG
Adapter-Index10	/5PHOS/GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCCGTCTTCTGCTTG
Adapter-Index11	/5PHOS/GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCCGTCTTCTGCTTG
Adapter-Index12	/5PHOS/GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTTCTGCTTG
Adapter-universal	AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATC*T
Reagent	Amount
Adapter-Universal oligo	20 μL
Adapter-Indexed oligo #	20 μL
Annealing Buffer (5×)	10 μL
TOTAL	50 μL