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. 2023 May 30;18(5):e0285597. doi: 10.1371/journal.pone.0285597

Fig 1. Addition of AM does not reduce the chemokine scavenging activities of ACKR3 in a ligand competition assay in ndLECs in vitro.

Fig 1

(A-D) ndLECs stimulated by overnight treatment with TNFα/IFNγ take up (A, B) CXCL12-AF647 or (C, D) the ACKR3-specific chimeric chemokine CXCL11/12-Atto565 at 37°C but not at 4°C. Uptake at 37°C is abrogated by treatment with CCX771. (A, C) Representative histograms and (B, D) quantifications of 3–5 independent experiments. One-way ANOVA. (E, F) Scavenging of the ACKR3-specific chimeric chemokine CXCL11/12-AF647 at 37°C by unstimulated and TNFα/IFNγ stimulated human LECs is abrogated by treatment with CCX771 (E) Representative histograms and (F) quantification of 3 independent experiments. Two-way ANOVA. (G) AM is not able to displace CXCL11/12-AF647 in a ligand competition assay. CXCL11/12-AF647 (50nM) scavenging assay was performed in presence of exceeding concentrations of AM. Data of three independent experiments are shown as mean ±SD. Each dot represents the value obtained in an individual experiment. Statistics: RM One-way ANOVA, Dunnett’s multiple comparison test.