Figure 3.
Responses of transgenic alfalfa lines carrying SBL to B. japonicum (p149). A, Methylene blue-stained root inoculated with USDA110 (p149); three regions indicating Ccds (*) are evident. Scale bar = 100 μm. B, Longitudinal section through a root with a region showing Ccd (*) developed in response to NodZ− (p149). Scale bar = 100 μm. C, Section through the edge of a root showing cortical cell expansion (*) and polar attachment of bradyrhizobia (arrowheads) to the epidermal and root hair cells. Scale bar = 10 μm. D, USDA110 (p149) cells are polarly attached to the root hairs and epidermal cells (arrowheads). Bradyrhizobia are also evident within cells (arrow) and intercellular spaces. Scale bar = 20 μm. E, Polar attachment of USDA110 (p149) cells to root hairs (arrowhead). Hyaline spots indicating the beginnings of bacterial penetration into the root hairs (arrows). Scale bar = 10 μm.