Table II.

Analysis of individual plants issued from the B31 × B10 cross at two stages of development

Age	Plant	CCR Relative Activity	COMT I Relative Activity	LK	Units Only Involved in β-O-4 Bonds
					%	S:G
				%
6 Weeks	Controls	100 ± 21	100 ± 8	11.8 ± 1.0	38.5 ± 3	0.9 ± 0.1
	A	10	32	10.7	7.2	1.7
	C	14	28	nd	nd	1.2
	E	2	38	11.0	35.2	0.8
	F	7	14	10.6	34.0	0.9
	G	nd	21	8.2	22.9	0.8
11 Weeks	Controls	100 ± 23	100 ± 8	15.3 ± 0.8	44.0 ± 5	1.2 ± 5
	H	14	36	8.4	18.2	2.3
	I	7	34	7.6	20.2	2.3
	J	nd	nd	7.0	19.2	2.1
	K	nd	32	8.3	15.2	2.7

Analyses were conducted on untransformed controls or double transformants that were 6 or 11 weeks old. COMT I and CCR activities are expressed relative to the controls. Klason lignin (LK) and thioacidolysis evaluation of lignin units involved only in β-O-4 bonds were determined as described in Table I. Nd, Not determined. Values of controls were calculated from the data of four or three plants for 6- and 11-week-old tobaccos, respectively. All measurements were duplicated.