Figure 3.

GA treatment of barley aleurone layers reduces their ability to tolerate H₂O₂. H₂O₂ (1% [v/v]; 325 mm) was added to aleurone layers incubated in ABA or GA for 12, 18, or 24 h. Layers were stained with FDA and FM 4-64 1 h after H₂O₂ addition and cells were scored live or dead. The data at each time point are from six aleurone layers, with two determinations per layer and approximately 150 cells per determination. Bars are the mean percentage of live cells in each determination ± sd from the mean.