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Effect of GA and ABA on the amount of Cat1 and Cat2 mRNA in aleurone. RNA was isolated from layers incubated in GA or ABA, or from layers preincubated in 5 μm ABA with the addition of 25 μm GA at 12 h. A, RNA gel blot was probed with barley Cat1 cDNA, Cat2 cDNA, Amy cDNA probes, and maize 28s rRNA probe. B, The abundance of each RNA was determined by laser densitometry (●, GA; ▪, ABA; ▴, 12 h ABA, then addition of 5-fold excess GA).
