Figure 2.

Transport of proricin to vacuoles is sensitive to BFA in tobacco leaf protoplasts. A, Protoplasts from tobacco leaves were transfected with the ppricin construct, pulse labeled for 1 h in the presence (+) or in the absence (-) of 10 μg mL⁻¹ of BFA, and chased for the indicated periods of time. Cell homogenates (lanes 1–6) and incubation media (lanes 7–12) were subjected to immunoprecipitation with rabbit anti-RTA antiserum and analyzed by SDS-PAGE and fluorography. White arrowheads indicate the position of proricin and black arrowheads indicate mature RTA and RTB. Numbers at right indicate molecular mass markers in kilodaltons.