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. 2023 May 30;12:e81646. doi: 10.7554/eLife.81646

Table 1. Simulation specifications.

The definition and values of the parameters used in the presented simulations are tabulated.

Parameters Description Values
Specification of catAb
K D Dissociation constant of antibody 10 nM
(KD)catenator Dissociation constant of catenator 10 nM-10 mM
[catAb] Antibody concentration 1 nM
l Length of the flexible linker 6 nm
c Length of the catenator 2 nm
L Reach length (l+c/2) 7 nm
Specification of the target surface
Ntotal_binding_sites
(in Figures 3 and 4)
Number of antibody-binding sites 98–102
Connectivity number
(in Figures 3 and 4)
Number of possible catenation 3 (Hexagonal)
4 (Square)
6 (Triangular)
d (in Figures 3 and 4) Distance between adjacent binding sites 12 nm
Lsurface (in Figure 4) Surface area of the target surface 40 nm2
Binding site density
(in Figure 4)
Surface density of the binding sites 1.47–11.76
(per 7x7 nm2)
Specification of simulation
Updates/MCMC step Number of updates in one MCMC step 30,000–100,000
Sampling size Number of sampling for a parameter set 1024