Appendix 1—key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Antibody | Anti-Brp (mouse monoclonal) | DSHB, University of Iowa, USA | DSHB Cat# nc82, RRID:AB_2314866 | 1/40 |
| Antibody | Anti-GFP (chicken polyclonal) | Thermo Fisher Scientific | Thermo Fisher Scientific Cat# A10262, RRID:AB_2534023 | 1/1000 |
| Antibody | Anti-chicken Alexa Fluor 488 (goat polyclonal) | Thermo Fisher Scientific | Thermo Fisher Scientific Cat# A11039, RRID:AB_2534096 | 1/500 |
| Antibody | Anti-mouse Alexa Fluor 647 (goat polyclonal) | Thermo Fisher Scientific | Thermo Fisher Scientific Cat# A21236, RRID:AB_2535805 | 1/500 |
| Antibody | Anti-HA (rabbit monoclonal) | Cell Signalling Technology | Cell Signaling Technology (C29F4) Cat #3724, RRID:AB_1549585 |
1/1000 |
| Antibody | Anti-rabbit Alexa Fluor 488 (goat polyclonal) | Thermo Fisher Scientific | Thermo Fisher Scientific Cat# A11034, RRID:AB_2576217 | 1/500 |
| Antibody | Anti-DYKDDDK epitope tag (rat monoclonal) | Novus Biologicals | Novus Biologicals, NBP1-06712, RRID:AB_1625981 | 1/1000 |
| Antibody | Anti-rat Alexa Fluor 568 (goat polyclonal) | Thermo Fisher Scientific | Thermo Fisher Scientific Cat# A11077, RRID#AB_2534121 | 1/500 |
| Antibody | Anti-dsRed (rabbit polyclonal) | Takara | Takara Bio Cat# 632496, RRID:AB_10013483 | 1/1000 |
| Antibody | Anti-rabbit Alexa Fluor 568 (goat polyclonal) | Thermo Fisher Scientific | Thermo Fisher Scientific Cat# A11011, RRID#AB_143157 | 1/500 |
| Chemical Compound, drug | All trans-Retinal | MilliporeSigma | Cat # R2500, CAS:116-31-4 | |
| Chemical Compound, drug | Sucrose | Thermo Fisher Scientific | Thermo Fisher Scientific:Cat# AAA1558336; CAS:57-50-1 |
|
| Genetic reagent (D. melanogaster) | Wild-type, Canton-S | Bloomington Stock Center | ||
| Genetic reagent (D. melanogaster) | Gr64f-Gal4 | Bloomington Stock Center | RRID: BDSC_57669 | |
| Genetic reagent (D. melanogaster) | Gr64f-Gal4 | Bloomington Stock Center | RRID: BDSC_57668 | |
| Genetic reagent (D. melanogaster) | UAS-GCaMP6s(attP40) | Bloomington Stock Center | RRID: BDSC_42746 | |
| Genetic reagent (D. melanogaster) | UAS-GCaMP6s(VK00005) | Bloomington Stock Center | RRID: BDSC_42749 | |
| Genetic reagent (D. melanogaster) | UAS-CD8-tdTomato | Thistle et al., 2012 | ||
| Genetic reagent (D. melanogaster) | ovoD1 | Mével-Ninio et al., 1996 | ||
| Genetic reagent (D. melanogaster) | hs-bam | Bloomington Stock Center | RRID: BDSC_24636 | |
| Genetic reagent (D. melanogaster) | nsyb-Gal4 | Bloomington Stock Center | RRID: BDSC_51941 | |
| Genetic reagent (D. melanogaster) | UAS-dcr-2 | Bloomington Stock Center | RRID: BDSC_24650 | |
| Genetic reagent (D. melanogaster) | UAS-SPR-IR1 | Yapici et al., 2008 | ||
| Genetic reagent (D. melanogaster) | ppk-gal4 | Bloomington Stock Center | RRID: BDSC_32079 | |
| Genetic reagent (D. melanogaster) | VT003280-gal4 (attP2) | Feng et al., 2014 | ||
| Genetic reagent (D. melanogaster) | VT003280-LexA (attP2) | Feng et al., 2014 | ||
| Genetic reagent (D. melanogaster) | FruP1-LexA | Mellert et al., 2010 | ||
| Genetic reagent (D. melanogaster) | 8xLexAop2-FLPL(attP40) | Bloomington Stock Center | RRID: BDSC_55820 | |
| Genetic reagent (D. melanogaster) | UAS >stop > KIR | Bloomington Stock Center | RRID: BDSC_67686 | |
| Genetic reagent (D. melanogaster) | UAS >stop > TNT | Bloomington Stock Center | RRID: BDSC_67690 | |
| Genetic reagent (D. melanogaster) | UAS >stop > CD8::GF | Bloomington Stock Center | RRID: BDSC_30125 | |
| Genetic reagent (D. melanogaster) | VT050405-p65ADZp(attP40) | Tirian and Dickson, 2017 | ||
| Genetic reagent (D. melanogaster) | dsx-ZpGal4DBD | Shirangi et al., 2016 | ||
| Genetic reagent (D. melanogaster) | pC1 split-GAL4, SS01491 | Bloomington Stock Center | RRID:BDSC_86830 | Full genotype: VT002064-p65ADZp(attP40); VT008469-ZpGal4DBD(attP2) |
| Genetic reagent (D. melanogaster) | VT002064-p65ADZp | Bloomington Stock Center | RRID:BDSC_72442 | |
| Genetic reagent (D. melanogaster) | vpo split-GAL4, SS50200 | Bloomington Stock Center | RRID:BDSC_86868 | Full genotype: 31D07-p65ADZp (attP40); 52F12-ZpGal4DBD (attP2) |
| Genetic reagent (D. melanogaster) | vpo split-GAL4, SS50795 | Bloomington Stock Center | RRID:BDSC_86869 | Full genotype: VT045670-p65ADZp (attP40); 52F12-ZpGal4DBD (attP2) |
| Genetic reagent (D. melanogaster) | oviIN split-GAL4, SS65422 |
Bloomington Stock Center | RRID:BDSC_86837 | Full genotype: 68A10-p65ADZp (attP40); VT010054-ZpGal4DBD (attP2) |
| Genetic reagent (D. melanogaster) | oviIN split-GAL4, SS65423 |
Bloomington Stock Center | RRID:BDSC_86838 | Full genotype: VT026347-p65ADZp (attP40); VT026035-ZpGal4DBD (attP2) |
| Genetic reagent (D. melanogaster) | oviDN split-GAL4, SS46540 |
Bloomington Stock Center | RRID:BDSC_86832 | Full genotype: VT050660-p65ADZp (attP40); VT028160-ZpGal4DBD (attP2) |
| Genetic reagent (D. melanogaster) | oviDN split-GAL4, SS35666 |
Bloomington Stock Center | RRID:BDSC_86831 | Full genotype: VT026873-p65ADZp (attP40); VT040574-ZpGal4DBD (attP2) |
| Genetic reagent (D. melanogaster) | 20xUAS-IVS-GtACR1-EYFP9attP2 | Mohammad et al., 2017 | ||
| Genetic reagent (D. melanogaster) | 20xUAS-CsChrimson::mVenus (attP18) | Bloomington Stock Center | RRID: BDSC_55134 | |
| Genetic reagent (D. melanogaster) | MCFO | Bloomington Stock Center, Nern et al., 2015 | RRID:BDSC_64088 | |
| Genetic reagent (D. melanogaster) | VT004974-p65ADZp (attP40) | Bloomington Stock Center | RRID: BDSC_71475 | |
| Genetic reagent (D. melanogaster) | R69H11-ZpGal4DBD (attP2) | Bloomington Stock Center | RRID: BDSC_69819 | |
| Genetic reagent (D. melanogaster) | VT023823-p65ADZp (attP40) | Tirian and Dickson, 2017 | ||
| Genetic reagent (D. melanogaster) | VT027804-p65ADZp (attP40) | Bloomington Stock Center | RRID: BDSC_72041 | |
| Genetic reagent (D. melanogaster) | VT057275-p65ADZp (attP40) | Bloomington Stock Center | RRID: BDSC_71664 | |
| Genetic reagent (D. melanogaster) | R9B05-p65ADZp (attP40) | Bloomington Stock Center | RRID: BDSC_70519 | |
| Genetic reagent (D. melanogaster) | R22D06-ZpGal4DBD (attP2) | Dionne et al., 2018 | ||
| Genetic reagent (D. melanogaster) | R29G03-p65ADZp (attP40) | Bloomington Stock Center | RRID: BDSC_75746 | |
| Genetic reagent (D. melanogaster) | R60C09-p65ADZp (attP40) | Bloomington Stock Center | RRID: BDSC_69750 | |
| Genetic reagent (D. melanogaster) | R19B09-gal4 (attP2) | Bloomington Stock Center | RRID: BDSC_48840 | |
| Genetic reagent (D. melanogaster) | R19B09-LexA (attP40) | Bloomington Stock Center | RRID: BDSC_52539 | |
| Genetic reagent (D. melanogaster) | UAS >stop > csChrimson w-,UAS >stop > csChrimson (attP18) | Wu et al., 2016 | ||
| Genetic reagent (D. melanogaster) | UAS-Chrimson88 | Klapoetke et al., 2014 | ||
| Genetic reagent (D. melanogaster) | LexAop-GCaMP6s | Broussard et al., 2018 | ||
| Genetic reagent (D. melanogaster) | R40F04-LexA (attP40) | Bloomington Stock Center | RRID: BDSC_54785 | |
| Software, Algorithm | Fiji | https://fiji.sc/ | RRID: SCR_002285 | |
| Software, Algorithm | GraphPad Prism | Graphpad Software; https://www.graphpad.com/scientific-software/prism/ | RRID:SCR_002798 | |
| Software, Algorithm | Python | Python Software Foundation; https://www.python.org/downloads/ | RRID:SCR_008394 | |
| Software, Algorithm | Flywire | Flywire; https://flywire.ai/ | RRID:SCR_019205 | |
| Software, Algorithm | Adobe Illustrator | Adobe Software; https://www.adobe.com/products/illustrator.html | RRID:SCR_010279 | |
| Software, Algorithm | CATMAID | Saalfeld et al., 2009; https://catmaid.org | RRID:SCR_006278 | |
| Software, Algorithm | CAVE (connectome annotation versioning engine) | Buhmann et al., 2021, Heinrich et al., 2018 | ||
| Software, Algorithm | R Project for Statistical Computing | Dessau and Pipper, 2008; www.r-project.org | RRID:SCR_001905 | |
| Software, Algorithm | SciPy package | Virtanen et al., 2020; https://scipy.org/ | RRID:SCR_008058 | |
| Software, Algorithm | CircuitCatcher | Bushey, 2019 | ||
| Other | FLIC | Ro et al., 2014; https://www.sablesys.com/products/classic-line/flic_drosophila_behavior_system/ | ||
| Peptide, recombinant protein | Alexa Fluor 633 Phalloidin | Thermo Fisher Scientific | Thermo Fisher Scientific Cat# A22284 | 1/500 |