TABLE 1.
Characterization of the affinity eluted samples from all process conditions of Figure 4 by total protein, ds-DNA and, respective reduction percentage from the clarified feedstock (total protein: 2.8 mg mL-1 and ds-DNA: 0.2 μg mL-1).
| Samples | Total Protein | Total ds-DNA | |||
|---|---|---|---|---|---|
| Residence time (s) | Load volume (mL) | Concentration in eluate (mg mL-1) | Reduction percentage (%) | Concentration in eluate (µg mL-1) | Reduction percentage (%) |
| 1.2 | 250 | 0.15 | 95 | 0.02 | 90 |
| 1.6 | 250 | 0.19 | 93 | 0.03 | 85 |
| 2.4 | 100 | 0.14 | 95 | 0.02 | 90 |
| 250 | 0.26 | 91 | 0.03 | 85 | |
| 450 | 0.22 | 92 | 0.02 | 90 | |
| 900 | 0.27 | 90 | 0.03 | 85 | |
| 4.8 | 250 | 0.34 | 88 | 0.04 | 80 |
| 400* | 0.61 ± 0.01 | 78 ± 1 | 0.03 ± 0.01 | 84 ± 4 | |
*Affinity eluates from reproducibility study (n=3).