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Lupin cotyledons transfer the sulfur atom from [35S]sulfate into protein. Cotyledons were dissected from developing narrow-leaf lupin seeds at an early (approximately 18 DAF, tracks 1 and 3) or late (approximately 35 DAF, tracks 2 and 4) stage of maturation. Total protein was visualized by Coomassie Blue staining (tracks 1 and 2) and proteins containing 35S were visualized by fluorography (tracks 3 and 4). Molecular mass marker sizes (×10−3) are shown at right.
